Eiken Chemical Co,. Ltd.
Eiken Chemical Co,. Ltd.
The principle of LAMP method 

About LAMP method

"LAMP" which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on the target gene and the reaction process proceeds at a constant temperature using strand displacement reaction. Amplification and detection of gene can be completed in a single step, by incubating the mixture of samples, primers, DNA polymerase with strand displacement activity and substrates at a constant temperature (about 65°C). It provides high amplification efficiency, with DNA being amplified 109-1010 times in 15-60 minutes. Because of its high specificity, the presence of amplified product can indicate the presence of target gene.

 Characteristics

 
There is no need for a step to denature double stranded into a single stranded form.
The whole amplification reaction takes place continuously under isothermal conditions.
The amplification efficiency is extremely high.
By designing 4 primers to recognize 6 distinct regions, the LAMP method is able to specifically amplify the target gene.
The total cost can be reduced, as LAMP dose not require special reagents or sophisticated equipments.
The amplified products have a structure consisting of alternately inverted repeats of the target sequence on the same strand.
Amplification can be done with RNA templates following the same procedure as with DNA templates, simply through the addition of reverse transcriptase.

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